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ELISA stands for enzyme linked immunosorbent assay. In short, it is an antibody test or a test for immune response to things attacking the body such as virus, bacteria and allergens. The test is done in an ELISA plate, also known as a 96-well plate or microplate. ELISA readers or micro plate readers do spectrophotometry; they emit light at one wave length, and measure the amount of light absorbed and reflected by an object such as a protein. A spectrophotometer measures ultraviolet and visible light.
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Microplate washers are used in many applications such as ELISA, cell-based assays and microsphere-based assays. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. Formats available are strip washers, full plate washers and combination washer-dispensers. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. Safety considerations should include aerosol protection and an emergency stop. Some labs may require washing for biomagnetic separation and vacuum filtration assays; interchangeable wash manifolds are available to suit a variety of washing applications. High throughput labs should consider the ability to integrate a washer into the current lab workflow, and the ability to upgrade when needed. From hand held washers to fully automated systems, there is a suitable microplate washer for every lab’s needs.